Insertion junction: CLIP2.038889_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038889
Chromosome:chromosome 12
Location:1615256
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g486950 CSV7 Chlamydomonas-specific family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTCGGAGAGGGTGGGGTCTGACGACCTGTGTCCGACGACACGCAGG
Internal bar code:GTCGGGTTTACTACTTGCTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4888
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGAGCTAGAGTCCAAGGG
Suggested primer 2:AACTTAGGAAACCGGAGCCG