Insertion junction: CLIP2.038890_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.038890
Chromosome:chromosome 10
Location:4357439
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g449950 (1 of 1) PTHR14593:SF5 - WD REPEAT-CONTAINING PROTEIN 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGATGACACGCATCGTGACACCCTGCGCTGCCACCGTCCCACCTCTC
Internal bar code:GCGCTCTATAACTGTGCTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:254
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTGTGTGTGTGTG
Suggested primer 2:GACTCCAACAACAAGCTGCG