| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038891 |
| Chromosome: | chromosome 16 |
| Location: | 4553033 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671350 | ARS2 | (1 of 2) IPR012083 - Arylsulfatase; Periplasmic arylsulfatase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGACGCTGTTGCTTGGGACACCGACCACTGCGCTGCGATGACTCACAG |
| Internal bar code: | AGACAAATGTGCTTTGATAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3692 |
| LEAP-Seq percent confirming: | 92.9293 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGGTCGAGTTGCAAGCG |
| Suggested primer 2: | GAGGGAGGCGATAAGAACGG |