| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038933 |
| Chromosome: | chromosome 3 |
| Location: | 7838116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202875 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTCGTCCAGGACCTGCTGCGCCCGCGCCTGCGCCCGCGCCTCCGCTA |
| Internal bar code: | TGGGATGGGGCTGGGGCTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5037 |
| LEAP-Seq percent confirming: | 89.3333 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAACACGTGGCAGAAGGG |
| Suggested primer 2: | GCCAGATACGACTTGCTCGA |