Insertion junction: CLIP2.038952_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.038952
Chromosome:chromosome 5
Location:2430930
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g238687 PHC35 Pherophorin-chlamydomonas homolog 35; (1 of 1) IPR011993//IPR024616 - Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) // Pherophorin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGGTTGGCAGCTGCGCGCCGCCATTGTTTTGGCAGCAGCACAGCTTTG
Internal bar code:CCCTTAATTTATACTTTTTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGTGTGTGTGTCGCCTC
Suggested primer 2:ATTACACACGGCCTGGTGAG