Insertion junction: CLIP2.038964_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038964
Chromosome:chromosome 9
Location:6038349
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412600 CYG52 (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCTCAGTTCTTGTTCTTGTTCTTGTTCTTGTTCTTGTTCTTGTTCTT
Internal bar code:TCGATTTGAGGCATATTGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1491
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACAGACAAACGGTTGC
Suggested primer 2:TGCTTGTGTGTGTGTGGGAT