Insertion junction: CLIP2.038964_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.038964
Chromosome:chromosome 9
Location:6038361
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412600 CYG52 (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCTCTTGGCCCCACCCCACCTGGGCGAGGCGGGGTCGTACAGGTTTG
Internal bar code:CAGCATTGTCACTTTGGAGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTGTGTGTGTGTGGGAT
Suggested primer 2:GGTGACAGACAAACGGTTGC