| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.038971 |
| Chromosome: | chromosome 1 |
| Location: | 4380879 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029800 | Coiled-Coil Protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCTGGACAGCATGATGGGCTGCGACCGAATAGCGGTGCTCAGCTCCA |
| Internal bar code: | ATAATTGGTATCGGTTAATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5608 |
| LEAP-Seq percent confirming: | 92.6471 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATTGCTTGTTCGTCGGCG |
| Suggested primer 2: | CTCCAACGCGTGCATACATG |