Insertion junction: CLIP2.038976_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038976
Chromosome:chromosome 11
Location:521682
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCACCACCACCACCAGCACCCCCGCCACTGCTCCTTCCTGTCCGCCCC
Internal bar code:TGGTTTCTGCCTGTAGCGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1021
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAACGTGCTGTTCAGGTGTG
Suggested primer 2:CCTGTGCTGCTTTTCCACAC