Insertion junction: CLIP2.038990_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038990
Chromosome:chromosome 1
Location:4290181
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g029050 (1 of 1) PF08513//PF10607 - LisH (LisH) // CTLH/CRA C-terminal to LisH motif domain (CLTH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCATCCCCCACCACCCTTGCGACACCGACCAATAGCGCGCCCCACCTC
Internal bar code:TCGCTGTGCGACCATTTGCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2653
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGACTGAGGCACGAAGAC
Suggested primer 2:GTGCATGGGTGTGTGTGAAC