Insertion junction: CLIP2.039001_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039001
Chromosome:chromosome 3
Location:5514186
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g184900 (1 of 1) IPR027417//IPR029060 - P-loop containing nucleoside triphosphate hydrolase // PIN domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGGTCCAGTGGCCACCTTGTCATCGCGCTTGGTCGTGTCCTTCCGCG
Internal bar code:ACCACTCTTGTAGAATCCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2761
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTAGCAGTAGTGGCCAGC
Suggested primer 2:ACACACAGACGCACGTACAT