Insertion junction: CLIP2.039019_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039019
Chromosome:chromosome 2
Location:2857728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094750 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTGCCCGTTGCTCTCTCGCGCGCGTAGGTTGCATTTGATACACTGTC
Internal bar code:AGTTGGTCAATGAGTTATTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4273
LEAP-Seq percent confirming:98.4252
LEAP-Seq n confirming:125
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:127

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGGCTGAATGTTAGAAGC
Suggested primer 2:GAAGGAAACACCGCAAACCC