Insertion junction: CLIP2.039038_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039038
Chromosome:chromosome 14
Location:1472728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g617350 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCAGTGCGGGTTAGGCACGCCGGTGGAGACCATCGCGGCGCAACACC
Internal bar code:CAGAATTCAGTTACTTCATGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1603
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AATAAGAGTGCGCGGTCCAA
Suggested primer 2:TCGTCGAACGTGTTTGGGAT