Insertion junction: CLIP2.039041_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.039041
Chromosome:chromosome 7
Location:4929832
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346698 PHC75 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAACAATCCGTGTGGGGTCAGCAATCCGAGCAAACGGATACATAGCAT
Internal bar code:ATCTATACGGAATCCTTCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1545
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGCTGGGCACTCCTGATA
Suggested primer 2:CTCTCGCTCATCCACGTACC