| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039046 |
| Chromosome: | chromosome 7 |
| Location: | 956281 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGCTCCACCACATCCCGCAACACGGCCTCTGGGGTGGCAGCCTCGA |
| Internal bar code: | AACTGGGTTAATGTCGGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3870 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGTCTGACGACGAACA |
| Suggested primer 2: | TCCACAAGTCCATGAGCACC |