| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.039061 |
| Chromosome: | chromosome 1 |
| Location: | 2203410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012350 | (1 of 1) K02331 - DNA polymerase phi [EC:2.7.7.7] (POL5, MYBBP1A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGGCATCGCTTGGCACTCCCAACAACCCACACCCATGTTGCAACGCT |
| Internal bar code: | GCTAGTGTGGATTGCGCATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1437 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCCCTGTTTGGTTGTG |
| Suggested primer 2: | CGTTTTGGAGCTGTGTGGTG |