Insertion junction: CLIP2.039084_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039084
Chromosome:chromosome 2
Location:2857345
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACAGGCTTGCGCGCCGTCAGTGCCTGGGAGGCGCCTGCGGCAGCCGGG
Internal bar code:ATCAAATATTATGCACGACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:4123
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGGCTGAATGTTAGAAGC
Suggested primer 2:ACAACTACTGTGGCGCATCA