Insertion junction: CLIP2.039103_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039103
Chromosome:chromosome 17
Location:4245210
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATCCACCCGTCGCCTCCACCCACGGCCACCCCACCCACCTGTCGCACG
Internal bar code:CGGGTCATGCGCGTGGGTACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1635
LEAP-Seq percent confirming:79.1667
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGTGCGTCTTAATCACCT
Suggested primer 2:CTGTCTAAATCGCGCACACG