| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039114 |
| Chromosome: | chromosome 12 |
| Location: | 3632621 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513350 | (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAGGGCTACGGGGCCGCATTCAATGCCTCCAGCCCCGCGCCCGAGC |
| Internal bar code: | ACACGTCCGTATTAGGAATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2915 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGCCGTCAACTCTGTC |
| Suggested primer 2: | CTTCAACGGATAGCGTTGCG |