Insertion junction: CLIP2.039118_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039118
Chromosome:chromosome 17
Location:5548295
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739550 CPLD12 Conserved in the Plant Lineage and Diatoms; (1 of 11) 2.7.4.3 - Adenylate kinase / Myokinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCTGCCAGGTGCGCCTCCCGCCAGGTGCCACGCCTGCTGCTGCCGTG
Internal bar code:ATAATGACATCACATGCAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:64.7059
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTATACAACTCCCCGCCC
Suggested primer 2:ACATCTGTCTAGCATCGCGG