Insertion junction: CLIP2.039118_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039118
Chromosome:chromosome 17
Location:5548295
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739550 CPLD12 Conserved in the Plant Lineage and Diatoms; (1 of 11) 2.7.4.3 - Adenylate kinase / Myokinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTCCCACGCTGGATGGCTTCCGCGGTCGCCTTCGCGGCCTGGTCGATG
Internal bar code:CGGTTGCATATCGATCCGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:850
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCTGTCTAGCATCGCGG
Suggested primer 2:GTGTATACAACTCCCCGCCC