| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.039156 |
| Chromosome: | chromosome 10 |
| Location: | 6320617 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGCGGCAGCCAGGGCCAGGTGGCGGCTAGCCAGCAGGTGGTGCAGG |
| Internal bar code: | ATGTTAAGAGGGATCATCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2099 |
| LEAP-Seq percent confirming: | 76.7442 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAAATACGGGCGGTCAGT |
| Suggested primer 2: | GGGTTCATGGTTATGGGGCA |