Insertion junction: CLIP2.039178_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.039178
Chromosome:chromosome 12
Location:4841224
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g523550 UMM11 UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF752 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTCGAACAGCGCCGCACTGGCGGGGTTCTGCCGCGCCACCTCCAGCAC
Internal bar code:GGCGTCCTTTCATTATAAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1937
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AACGGTTTACACAACACGCG
Suggested primer 2:GCGTCTGCTTGTGTGTGTTT