| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.039187 |
| Chromosome: | chromosome 16 |
| Location: | 6556466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674200 | (1 of 3) PF07386 - Protein of unknown function (DUF1499) (DUF1499) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGTCGTCGAAGCTGGAGAGGCAAGTGTCTAGCCCCGCGCAGCTGTG |
| Internal bar code: | AGATGGAAACGAGGAGAAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3277 |
| LEAP-Seq percent confirming: | 83.0508 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGGTTCCTGGTGACCC |
| Suggested primer 2: | CATGTACCGCATCAGCAAGC |