| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039195 |
| Chromosome: | chromosome 2 |
| Location: | 1057300 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080300 | (1 of 5) PTHR10791 - RAG1-ACTIVATING PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGGCAGCACCAACTGCACAGGAGGGAGGGGCTTTTTAATGAAACAT |
| Internal bar code: | GCGGCGGCACTTTTTTTTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2503 |
| LEAP-Seq percent confirming: | 89.1304 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCGGTCACGTTGAGTAG |
| Suggested primer 2: | AGCTGTGCCCATGTCCATAC |