Insertion junction: CLIP2.039199_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.039199
Chromosome:chromosome 13
Location:688805
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g566350 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATAGTGATTGTGTTGGAGAGCAAAGGGTAGATAGGGAATGCGTGTGCG
Internal bar code:AGCGCTACGATACTGTGCCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:440
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCGCTCACGTTGTGAT
Suggested primer 2:CCCTCTCTACCCAGCTGTCT