Insertion junction: CLIP2.039199_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.039199
Chromosome:chromosome 13
Location:688807
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g566350 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTCACAGACACACAGACACACAAACACACGCACACACACAGACTGGCA
Internal bar code:AGCGCTACGATACTGTGCCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1251
LEAP-Seq percent confirming:43.6364
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCTCTACCCAGCTGTCT
Suggested primer 2:AAGTCCGCTCACGTTGTGAT