| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039220 |
| Chromosome: | chromosome 6 |
| Location: | 1823368 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263602 | PEX12 | Peroxin 12; (1 of 1) K13345 - peroxin-12 (PEX12, PAF3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTGTGGAGTCTGGGGATGACGGACACCCGTGCCCCAAACACCCGGGC |
| Internal bar code: | GTAGTTTTTATCCAAGGTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3218 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTCTCGGACAGCCTTT |
| Suggested primer 2: | GTTTGGCCTTTTCCACGTCC |