| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039220 |
| Chromosome: | chromosome 12 |
| Location: | 4715495 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522750 | GT90F22,GT90-22 | GT90 family protein 22; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATGCGCCTCGACCGCACCTGCCGCGGCGCGCAGAACCCCACCCGCA |
| Internal bar code: | TTTGGGTAATGCTCCGTCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2737 |
| LEAP-Seq percent confirming: | 88.0597 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGGGGAATGAAGGTGG |
| Suggested primer 2: | CCGTCGACCCTAGCACATAC |