| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.039223 |
| Chromosome: | chromosome 3 |
| Location: | 8890425 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205361 | 3'UTR | ||
| Cre03.g205473 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTTCAGCGCCGTGCGCGTGCCCCATCCCGCAAGCCTACCTAACTCCCG |
| Internal bar code: | TAACTAACGCATATAATCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 4.54545 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCACACACACACACACAC |
| Suggested primer 2: | AGGCTACCAATGGCAAGCAT |