| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039227 |
| Chromosome: | chromosome 7 |
| Location: | 2279264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327750 | TRP3 | Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAGATGAGGGCGCGCAGCACCGGCGTGCCGAACACGCTGGGGTGCAC |
| Internal bar code: | GTGATCCAAGCTGGTGACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1228 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTAAGTGCGTGCATGGC |
| Suggested primer 2: | ATGTGGTCCACGGTGATGAC |