| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039232 |
| Chromosome: | chromosome 12 |
| Location: | 6964501 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561100 | (1 of 1) PF00226//PF06839 - DnaJ domain (DnaJ) // GRF zinc finger (zf-GRF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCATAGCTTGGCATCTAAGCAGGTACCGTCCGCCAGCACCACGAGCA |
| Internal bar code: | AGGGTATTGATTACCGTTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3523 |
| LEAP-Seq percent confirming: | 89.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGAGTCTGCAACTGACC |
| Suggested primer 2: | CGTTCTCATCCGCAATGCTG |