Insertion junction: CLIP2.039232_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.039232
Chromosome:chromosome 12
Location:6964518
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g561100 (1 of 1) PF00226//PF06839 - DnaJ domain (DnaJ) // GRF zinc finger (zf-GRF) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTGTACGCTGGTGATGTGTGGTTTCGGGGGAGGCTTTGCCACGCGGG
Internal bar code:TCTACTCCAAGTGAGGAGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3310
LEAP-Seq percent confirming:93.1818
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTCTCATCCGCAATGCTG
Suggested primer 2:AGCTGAGTCTGCAACTGACC