Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.039239 |
Chromosome: | chromosome 16 |
Location: | 5358860 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g683450 | AGP2 | (1 of 4) 2.7.7.27 - Glucose-1-phosphate adenylyltransferase / ADP-glucose synthase; ADP-glucose pyrophosphorylase large subunit | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCGTTCCTTAGCACACACGGTATGCGCTACGTGATATGCAGCCATG |
Internal bar code: | GTAAGGCATGTACCGTGGGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1586 |
LEAP-Seq percent confirming: | 67.3469 |
LEAP-Seq n confirming: | 33 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCCATCAACTCGCCTTTCT |
Suggested primer 2: | TACACGCCCTCCTTGTTGAC |