Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.039261 |
Chromosome: | chromosome 6 |
Location: | 1100293 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g257600 | FA1 | (1 of 4) PF13516 - Leucine Rich repeat (LRR_6); Flagellar Autonomy Protein | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGCTGCAGCCCCGCCGCCGCTGCCACGCCACTGCCGGCCGCAGGCG |
Internal bar code: | CTTACCCTCTTAAAACTCTAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1885 |
LEAP-Seq percent confirming: | 88.8889 |
LEAP-Seq n confirming: | 24 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTAGGTGCGAATGATTGCG |
Suggested primer 2: | GGAGATGGTGATGTTGGCCA |