| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039266 |
| Chromosome: | chromosome 12 |
| Location: | 1600981 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487101 | CSV9 | Chlamydomonas-specific family protein; (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGCGCAGGGTGGCGGCTGGGAGTTGGAGCTCGGGTGGGAGGGGCCC |
| Internal bar code: | ATTTCGCTGTGCCTTGTGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4251 |
| LEAP-Seq percent confirming: | 87.6404 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTTGCAAATGCCAGCAC |
| Suggested primer 2: | TGTCCTCAACCCATCAAGCC |