| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039268 |
| Chromosome: | chromosome 8 |
| Location: | 4463876 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAAGGCTCACGCCCTTCTTTCATGCATCATGCATACCCGCTGAAAAC |
| Internal bar code: | GAGGGGTTTTAGTTAATTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4117 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGCAGGAGCCAGTGTC |
| Suggested primer 2: | GGGATGGATATGCCCCACTG |