Insertion junction: CLIP2.039290_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.039290
Chromosome:chromosome 3
Location:5300112
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g182650 PGA1 Phospholipid/glycerol acyltransferase; (1 of 2) K13511 - monolysocardiolipin acyltransferase (TAZ) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGGAAGGTTGCCGCGGCGCACCGTGGCGATGGCGCGGGCGAGGCGGG
Internal bar code:CACAGATTTTTGTGGTCTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:4114
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACTCACACGAAGGAGGC
Suggested primer 2:AACAAAACCCGCTGCATCAC