Insertion junction: CLIP2.039297_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039297
Chromosome:chromosome 6
Location:6724765
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCCTTCTCCCCCTTCTCTCTCTTACCTCCTATCCCCACACCATACC
Internal bar code:TTAGCCTGATTAACGGAAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2667
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:88
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:88

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGCTCGGGGTGTATACG
Suggested primer 2:AAGACAGTGTCTGCGATGCA