| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039298 |
| Chromosome: | chromosome 16 |
| Location: | 7739136 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686846 | (1 of 1) PTHR22797//PTHR22797:SF40 - CARD6/NUCLEOLAR PROTEIN 3 // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGGGGCAGCGCGCCCCTGCGGGAAACGTCTGTGGGATGCAGCAGCT |
| Internal bar code: | GTTTGTTAAGTGAGGTTTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1474 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCGACAACAGTTTTGGC |
| Suggested primer 2: | CCGTATGAGATCACCCTGGC |