| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039322 |
| Chromosome: | chromosome 5 |
| Location: | 1549996 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234665 | AP4B4 | Beta4-Adaptin; (1 of 1) PTHR11134:SF4 - AP-4 COMPLEX SUBUNIT BETA-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCCTCAGGGGTGGGGGGGAGTGACACCTTTGACGCATGCTAGCGCG |
| Internal bar code: | AGGGGTGAGGGCGGAGTTACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1326 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGGTGCGTGAAACAGG |
| Suggested primer 2: | TTAATAGGTGGACGTGGCGG |