| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039349 |
| Chromosome: | chromosome 11 |
| Location: | 1786953 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467800 | (1 of 1) PF06545 - Protein of unknown function (DUF1116) (DUF1116) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCAAGCCTGGCGGTGACGCAAGCACTCCCGCGACTCGCACGCAGTGA |
| Internal bar code: | TGTAGGCATCGTCGAGCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3279 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTGATGGTTGGATGCCA |
| Suggested primer 2: | TTGTCGCCTCAACTGGGATC |