Insertion junction: CLIP2.039360_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.039360
Chromosome:chromosome 16
Location:7576746
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683931 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCTGCATTATCGCGAATCGTCTCCGACCCCCCCCGCACCTGCATCAGC
Internal bar code:CAGGTTAACCTGGCTTTTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1389
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCAAAGCCCCTACACTA
Suggested primer 2:ACATGGTCGCCGAGAGTTTT