Insertion junction: CLIP2.039396_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.039396
Chromosome:chromosome 2
Location:2851620
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACTGAGGTGCAGAATGGTGGCAAGGTTGCTCGCACCACGGTCATGATG
Internal bar code:GTCTTTAGTATTGGCTTGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:5699
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTGCACCATGATGATC
Suggested primer 2:GGTTCCAACCTCTGCTTCGA