Insertion junction: CLIP2.039399_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.039399
Chromosome:chromosome 10
Location:3996588
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g447300 DDI1 DNA-damage inducible protein 1 homolog; (1 of 1) K11885 - DNA damage-inducible protein 1 (DDI1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAGGGCTGCTAGGTGGCTGCGTTTGGCTCGGCGCGGCGGCGCTGGGCA
Internal bar code:GAGTGAGCGAATCCGGTGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:220
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATGTAACGACGGATGCC
Suggested primer 2:CAACCACCAAAACGTCGACC