| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.039410 |
| Chromosome: | chromosome 16 |
| Location: | 1045400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGTAT |
| Internal bar code: | GTTATAAGACGTGTGCTGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 421 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCCGTTGTATGCTGC |
| Suggested primer 2: | CGTGTGCTCCTTTGTGTGTG |