| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.039418 |
| Chromosome: | chromosome 2 |
| Location: | 6446140 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388500 | (1 of 13) PF09273 - Rubisco LSMT substrate-binding (Rubis-subs-bind) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGGACTCTGCGTCGTCCTGCCTGACCTGCTGTGTGGTGTGCCCTGT |
| Internal bar code: | GTGCGCGGTATGTTCCTCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1777 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGAGGAGATTCAGACCG |
| Suggested primer 2: | GCATATGCAGCCGTTGGAAG |