Insertion junction: CLIP2.039436_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.039436
Chromosome:chromosome 2
Location:4676558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g107800 CNK9 NimA-related protein kinase; (1 of 15) K08857 - NIMA (never in mitosis gene a)-related kinase [EC:2.7.11.1] (NEK) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCGCCGACAGTTGCTGTCACAGTAGGGGGACCTGTCATTGCGGCGGC
Internal bar code:GCCTGCTCCAGCCGTTTCATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1802
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGCAAAGCTAGCACCGC
Suggested primer 2:CGTCGCTGTATCGTCCATGA