| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.039449 |
| Chromosome: | chromosome 17 |
| Location: | 5935982 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742050 | (1 of 18) IPR011051 - RmlC-like cupin domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTCGGCCAGGGGCAGTCCCGCCTCGATCATGCTGCGGTACGGCTTGT |
| Internal bar code: | CTGAGCTATGGTGCTTTTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3194 |
| LEAP-Seq percent confirming: | 91.4286 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAGGGAGGGAGGGAAAT |
| Suggested primer 2: | GTCTACAGCGAGCGACTCTC |