Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.039454 |
Chromosome: | chromosome 3 |
Location: | 2161825 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g157000 | ELG1 | Exostosin-like glycosyltransferase 1; (1 of 1) PF12661 - Human growth factor-like EGF (hEGF) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCTTAGCTGACCTTGCCCACCTGGGCACGACTGCGTAATCTATGGT |
Internal bar code: | ATAGTTGTAGCAGGGGCAATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 4587 |
LEAP-Seq percent confirming: | 98.8235 |
LEAP-Seq n confirming: | 84 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACCTCACAGCATGCCTACC |
Suggested primer 2: | TTGGATGGACATCACACGGG |